CD112 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2679R-BF647 |
| Product Name | CD112 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human nectin 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5819 |
| Swiss Prot | Q92692 |
| Subcellular location | Cell membrane |
| Synonyms | HVEB; PRR2; CD112; PVRR2; Nectin-2; Herpes virus entry mediator B; Herpesvirus entry mediator B; Poliovirus receptor-related protein 2; PVRL2 |
| Background | Nectin 2 is a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Can form trans-heterodimers with PVRL3/nectin-3. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD112 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-2679R-BF647) validated for, and at what dilution?
bs-2679R-BF647 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD112 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-2679R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Horse.
How should bs-2679R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD112 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-2679R-BF647)?
KLH conjugated synthetic peptide derived from human nectin 2
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-2679R-BF647)?
AbBy Fluor-647 on bs-2679R-BF647: excitation / emission 650nm/665nm.