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GPNMB Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Cow
  • Pig
  • Horse
  • GuineaPig
Overview
Catalog # bs-2684R-Biotin-100uL
Product Name GPNMB Polyclonal Antibody, Biotin Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Human, Rat
Predicted Reactivity Mouse, Cow, Pig, Horse, GuineaPig
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GPNMB
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 10457
Swiss Prot Q14956
Subcellular location Cell membrane
Synonyms NMB; HGFIN; Transmembrane glycoprotein NMB; Transmembrane glycoprotein HGFIN; GPNMB; UNQ1725/PRO9925
Background The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is GPNMB Polyclonal Antibody, Biotin Conjugated (bs-2684R-Biotin) validated for, and at what dilution?

bs-2684R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GPNMB Polyclonal Antibody, Biotin Conjugated (bs-2684R-Biotin) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Pig, Horse, GuineaPig.

How should bs-2684R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GPNMB Polyclonal Antibody, Biotin Conjugated (bs-2684R-Biotin)?

KLH conjugated synthetic peptide derived from human GPNMB

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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