GPNMB Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2684R-APC |
| Product Name | GPNMB Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GPNMB |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10457 |
| Swiss Prot | Q14956 |
| Subcellular location | Cell membrane |
| Synonyms | NMB; HGFIN; Transmembrane glycoprotein NMB; Transmembrane glycoprotein HGFIN; GPNMB; UNQ1725/PRO9925 |
| Background | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GPNMB Polyclonal Antibody, APC Conjugated (bs-2684R-APC) validated for, and at what dilution?
bs-2684R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPNMB Polyclonal Antibody, APC Conjugated (bs-2684R-APC) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Pig, Horse, GuineaPig.
How should bs-2684R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPNMB Polyclonal Antibody, APC Conjugated (bs-2684R-APC)?
KLH conjugated synthetic peptide derived from human GPNMB
What are the excitation and emission wavelengths of the APC conjugate (bs-2684R-APC)?
APC on bs-2684R-APC: excitation / emission 650nm/660nm.