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IL27R Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
Overview
Catalog # bs-2711R-FITC
Product Name IL27R Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IL-27RA
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9466
Swiss Prot Q6UWB1
Subcellular location Cell membrane
Synonyms CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1; Interleukin-27 receptor subunit alpha; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; Cytokine receptor WSX-1; Cytokine receptor-like 1; Type I T-cell cytokine receptor; IL27RA; UNQ296/PRO336
Background Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is IL27R Polyclonal Antibody, FITC Conjugated (bs-2711R-FITC) validated for, and at what dilution?

bs-2711R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IL27R Polyclonal Antibody, FITC Conjugated (bs-2711R-FITC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.

How should bs-2711R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IL27R Polyclonal Antibody, FITC Conjugated (bs-2711R-FITC)?

KLH conjugated synthetic peptide derived from human IL-27RA

What are the excitation and emission wavelengths of the FITC conjugate (bs-2711R-FITC)?

FITC on bs-2711R-FITC: excitation / emission 494nm/518nm.