p53BP1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2740R-Cy5.5-100uL |
| Product Name | p53BP1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human p53BP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7158 |
| Subcellular location | Nucleus |
| Synonyms | 53 BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; p53BP1; TP53 BP1; TP53BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; TP53B_HUMAN; tumor protein 53-binding protein, 1. |
| Background | p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double strand breaks. This also suggests a role in DNA repair, maintaining genomic stability. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is p53BP1 Polyclonal Antibody, Cy5.5 Conjugated (BS-2740R-CY5-5) validated for, and at what dilution?
BS-2740R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p53BP1 Polyclonal Antibody, Cy5.5 Conjugated (BS-2740R-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Rabbit.
How should BS-2740R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p53BP1 Polyclonal Antibody, Cy5.5 Conjugated (BS-2740R-CY5-5)?
KLH conjugated synthetic peptide derived from human p53BP1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-2740R-CY5-5)?
Cy5.5 on BS-2740R-CY5-5: excitation / emission 675nm/694nm.