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Aurora C Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-2750R-PE-Cy5-100uL
Product Name Aurora C Polyclonal Antibody, PE-Cy5 Conjugated
Applications
WB
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Pig, Horse, Rabbit
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Aurora C
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6795
Subcellular location Cytoplasm, Nucleus
Synonyms AURKC; AIE2; AIK3; AurC; AURKC; Aurora C; Aurora/Ipl1 related kinase 3; IPL-1; Aurora/Ipl1/Eg2 protein 2; EC 2.7.11.1; Serine threonine protein kinase 13; STK13; AURKC_HUMAN.
Background The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Aurora C Polyclonal Antibody, PE-Cy5 Conjugated (bs-2750R-PE-Cy5) validated for, and at what dilution?

bs-2750R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Aurora C Polyclonal Antibody, PE-Cy5 Conjugated (bs-2750R-PE-Cy5) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Pig, Horse, Rabbit.

How should bs-2750R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Aurora C Polyclonal Antibody, PE-Cy5 Conjugated (bs-2750R-PE-Cy5)?

KLH conjugated synthetic peptide derived from human Aurora C

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-2750R-PE-Cy5)?

PE-Cy5 on bs-2750R-PE-Cy5: excitation / emission 488nm/670nm.

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