CSF1R Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2755R-PerCP-Cy5.5-100uL |
| Product Name | CSF1R Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MCSF receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1436 |
| Swiss Prot | P07333 |
| Subcellular location | Cell membrane |
| Synonyms | FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R; Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; Proto-oncogene c-Fms; CSF1R; CSF1R_HUMAN |
| Background | The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CSF1R Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-2755R-PERCP-CY5-5) validated for, and at what dilution?
BS-2755R-PERCP-CY5-5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CSF1R Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-2755R-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should BS-2755R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CSF1R Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-2755R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human MCSF receptor
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-2755R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-2755R-PERCP-CY5-5: excitation / emission 482nm/694nm.