DARPP32 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2762R-FITC |
| Product Name | DARPP32 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat, GuineaPig |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DARPP32 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84152 |
| Swiss Prot | Q9UD71 |
| Subcellular location | Cytoplasm |
| Synonyms | DARPP32; DARPP-32; Protein phosphatase 1 regulatory subunit 1B; Dopamine- and cAMP-regulated neuronal phosphoprotein; PPP1R1B |
| Background | This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DARPP32 Polyclonal Antibody, FITC Conjugated (bs-2762R-FITC) validated for, and at what dilution?
bs-2762R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DARPP32 Polyclonal Antibody, FITC Conjugated (bs-2762R-FITC) react with?
Tested reactivity: Rat, GuineaPig. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Rabbit.
How should bs-2762R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DARPP32 Polyclonal Antibody, FITC Conjugated (bs-2762R-FITC)?
KLH conjugated synthetic peptide derived from human DARPP32
What are the excitation and emission wavelengths of the FITC conjugate (bs-2762R-FITC)?
FITC on bs-2762R-FITC: excitation / emission 494nm/518nm.