P105 RB Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-P105 RB Polyclonal Antibody, Unconjugated (bs-2777R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-P105 RB Polyclonal Antibody, Unconjugated (bs-2777R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Lane 1: Mouse Thymus tissue lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human Jurkat cell lysates probed with Rb Polyclonal Antibody, Unconjugated (bs-2777R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Thymus tissue lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human Jurkat cell lysates probed with Rb Polyclonal Antibody, Unconjugated (bs-2777R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-2777R
Product Name	P105 RB Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Rb/P105 RB
Immunogen Range	51-150/928
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5925
Swiss Prot	P06400
Subcellular location	Nucleus
Synonyms	RB; pRb; OSRC; pp11; p15-Rb; PPP1R13; Retinoblastoma-associated protein; RB1
Background	Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV42H1 and SUV42H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-2' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV4 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Predicted Reactivity

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