Claudin 4 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2790R-APC-Cy5.5-100uL |
| Product Name | Claudin 4 Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CLDN4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1364 |
| Swiss Prot | O14493 |
| Subcellular location | Cell membrane |
| Synonyms | CPER; CPE-R; CPETR; CPETR1; WBSCR8; hCPE-R; Claudin-4; Clostridium perfringens enterotoxin receptor; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein; CLDN4 |
| Background | Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact made up of network of strands that act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Claudin 4 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-2790R-APC-CY5-5) validated for, and at what dilution?
BS-2790R-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Claudin 4 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-2790R-APC-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should BS-2790R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Claudin 4 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-2790R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human CLDN4
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-2790R-APC-CY5-5)?
APC-Cy5.5 on BS-2790R-APC-CY5-5: excitation / emission 650nm/694nm.