RGS5 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2794R-Cy5 |
| Product Name | RGS5 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody may have a minor secondary cross-reactivity to the related proteins RGS3 and RGS4 based on a 67% and 73% non-sequential sequence similarity, respectively. |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse RGS5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 19737 |
| Swiss Prot | O08850 |
| Subcellular location | Cytoplasm |
| Synonyms | 1110070A02Rik; Regulator of G-protein signaling 5; RGS5 |
| Background | Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RGS5 Polyclonal Antibody, Cy5 Conjugated (bs-2794R-Cy5) validated for, and at what dilution?
bs-2794R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RGS5 Polyclonal Antibody, Cy5 Conjugated (bs-2794R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Pig, Rabbit.
How should bs-2794R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RGS5 Polyclonal Antibody, Cy5 Conjugated (bs-2794R-Cy5)?
KLH conjugated synthetic peptide derived from mouse RGS5
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-2794R-Cy5)?
Cy5 on bs-2794R-Cy5: excitation / emission 625,650nm/670nm.