SMO Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2801R-100uL |
| Product Name | SMO Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Smoothened |
| Immunogen Range | 551-650/787 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 6608 |
| Swiss Prot | Q99835 |
| Subcellular location | Cell membrane |
| Synonyms | Gx; SMOH; FZD11; Smoothened homolog; SMO; Protein Gx |
| Background | G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is SMO Polyclonal Antibody (bs-2801R) validated for, and at what dilution?
bs-2801R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMO Polyclonal Antibody (bs-2801R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Horse, Rabbit.
How should bs-2801R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMO Polyclonal Antibody (bs-2801R)?
KLH conjugated synthetic peptide derived from human Smoothened Immunogen range: 551-650/787.