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HDAC5 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat lung labeled with Anti-HDAC5 Polyclonal Antibody, Unconjugated (bs-2810R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat lung labeled with Anti-HDAC5 Polyclonal Antibody, Unconjugated (bs-2810R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen tissue labeled with Anti-HDAC5 Polyclonal Antibody, Unconjugated (bs-2810R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen tissue labeled with Anti-HDAC5 Polyclonal Antibody, Unconjugated (bs-2810R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human brain labeled with Anti-HDAC5 Polyclonal Antibody, Unconjugated (bs-2810R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human brain labeled with Anti-HDAC5 Polyclonal Antibody, Unconjugated (bs-2810R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with HDAC5 Polyclonal Antibody(bs-2810R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with HDAC5 Polyclonal Antibody(bs-2810R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
Overview
Catalog # bs-2810r
Product Name HDAC5 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF FLOW-CYT
Reactivity Human, Mouse, Rat, Bovine
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HDAC5
Immunogen Range 740-780/1122
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 9759, 10014
Swiss Prot P56524, Q9UQL6
Subcellular location Cytoplasm, Nucleus
Synonyms HDAC4: Histone deacetylase 4, HD4 HDAC 5; Histone deacetylase 5, HD5, Antigen NY-CO-9
Background Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]..
Application Dilution
WB
IHC-P
IHC-F
IF
Flow-Cyt

Frequently asked questions

Which applications is HDAC5 Polyclonal Antibody (bs-2810R) validated for, and at what dilution?

bs-2810R is validated for WB, IHC-P, IHC-F, IF, Flow-Cyt.

Which species does HDAC5 Polyclonal Antibody (bs-2810R) react with?

Tested reactivity: Human, Mouse, Rat, Bovine.

How should bs-2810R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HDAC5 Polyclonal Antibody (bs-2810R)?

KLH conjugated synthetic peptide derived from human HDAC5 Immunogen range: 740-780/1122.

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