SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T221) Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2854R-PerCP |
| Product Name | SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T221) Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T221) |
| Modification Site | SIK1+ SIK2+ SIK3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 150094 |
| Swiss Prot | P57059 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MSK; myocardial SNF1 like kinase; Salt inducible kinase 1; Salt-inducible protein kinase 1; Serine threonine protein kinase SNF1 like kinase 1; Serine threonine protein kinase SNF1LK; Serine/threonine-protein kinase SIK1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK; SIK-1; SIK1; SIK1_HUMAN; SNF1 like kinase. |
| Background | Serine/threonine-protein kinase involved in variousprocesses such as cell cycle regulation, gluconeogenesis andlipogenesis regulation, muscle growth and differentiation and tumorsuppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1,TORC1/CRTC1 and TORC2/CRTC2. Acts as a tumor suppressor and plays akey role in p53/TP53-dependent anoikis, a type of apoptosistriggered by cell detachment: required for phosphorylation ofp53/TP53 in response to loss of adhesion and is able to suppressmetastasis. Part of a sodium-sensing signaling network, probably bymediating phosphorylation of PPME1: following increases inintracellular sodium, SIK1 is activated by CaMK1 and phosphorylatesPPME1 subunit of protein phosphatase 2A (PP2A), leading todephosphorylation of sodium/potassium-transporting ATPase ATP1A1and subsequent increase activity of ATP1A1. Acts as a regulator ofmuscle cells by phosphorylating and inhibiting class II histonedeacetylases HDAC4 and HDAC5, leading to promote expression of MEF2target genes in myocytes. Also required during cardiomyogenesis byregulating the exit of cardiomyoblasts from the cell cycle viadown-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepaticgluconeogenesis by phosphorylating and repressing the CREB-specificcoactivators TORC1/CRTC1 and TORC2/CRTC2, leading to inhibit CREBactivity. Also regulates hepatic lipogenesis by phosphorylating andinhibiting SREBF1. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T221) Polyclonal Antibody, PerCP Conjugated (bs-2854R-PerCP) validated for, and at what dilution?
bs-2854R-PerCP is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T221) Polyclonal Antibody, PerCP Conjugated (bs-2854R-PerCP) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Chicken.
How should bs-2854R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T221) Polyclonal Antibody, PerCP Conjugated (bs-2854R-PerCP)?
KLH conjugated synthetic peptide derived from human SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T221)
What are the excitation and emission wavelengths of the PerCP conjugate (bs-2854R-PerCP)?
PerCP on bs-2854R-PerCP: excitation / emission 482nm/677nm.