HDAC7 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2890R-Cy3-100uL |
| Product Name | HDAC7 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HDAC7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | HD 7a; HD7a; HDAC 7; HDAC 7A; HDAC7; HDAC7A; Histone deacetylase 7; Histone deacetylase 7A; DKFZP586J0917; OTTHUMP00000202813; OTTHUMP00000202814; FLJ99588; HDAC7_HUMAN. |
| Background | HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | |
Frequently asked questions
Which applications is HDAC7 Polyclonal Antibody, Cy3 Conjugated (bs-2890R-Cy3) validated for, and at what dilution?
bs-2890R-Cy3 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HDAC7 Polyclonal Antibody, Cy3 Conjugated (bs-2890R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-2890R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HDAC7 Polyclonal Antibody, Cy3 Conjugated (bs-2890R-Cy3)?
KLH conjugated synthetic peptide derived from human HDAC7
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2890R-Cy3)?
Cy3 on bs-2890R-Cy3: excitation / emission 512,550nm/570,615nm.