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HDAC10 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-2893R-Cy5.5-100uL
Product Name HDAC10 Polyclonal Antibody, Cy5.5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Pig, Horse, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HDAC10
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 83933
Swiss Prot Q969S8
Subcellular location Cytoplasm, Nucleus
Synonyms HD10; Histone deacetylase 10; HDAC10
Background Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is HDAC10 Polyclonal Antibody, Cy5.5 Conjugated (BS-2893R-CY5-5) validated for, and at what dilution?

BS-2893R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HDAC10 Polyclonal Antibody, Cy5.5 Conjugated (BS-2893R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Rabbit.

How should BS-2893R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HDAC10 Polyclonal Antibody, Cy5.5 Conjugated (BS-2893R-CY5-5)?

KLH conjugated synthetic peptide derived from human HDAC10

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-2893R-CY5-5)?

Cy5.5 on BS-2893R-CY5-5: excitation / emission 675nm/694nm.