LC3B Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2912r-cy7-100uL |
| Product Name | LC3B Polyclonal Antibody, Cy7 Conjugated |
| Applications |
IF
|
| Reactivity | Mouse, Rat, Rabbit |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse, Chicken, Zebrafish |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MAP1A/MAP1B LC3 B |
| Immunogen Range | 21-121/121 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81631, 84557 |
| Subcellular location | Cytoplasm |
| Synonyms | MAP LC3 beta; LC3; Autophagy related protein LC3 A; Autophagy related protein LC3 B; Autophagy related ubiquitin like modier LC3 A; Autophagy related ubiquitin like modier LC3 B; MAP1 light chain 3 like protein 1; MAP1 light chain 3 like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1ALC3; MAP1BLC3; MAP1LC3A; MAP1LC3B; Microtubule associated protein 1 light chain 3 alpha; Microtubule associated protein 1 light chain 3 beta; Microtubule associated proteins 1A/1B light chain 3; Microtubule associated proteins 1A/1B light chain 3A; Microtubule associated proteins 1A/1B light chain 3B.microtubule-associated proteins 1A/1B light chain 3 beta 2 |
| Background | A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. |
| Application Dilution | |
| IF | 1:100-500 |
Frequently asked questions
Which applications is LC3B Polyclonal Antibody, Cy7 Conjugated (bs-2912R-Cy7) validated for, and at what dilution?
bs-2912R-Cy7 is validated for IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LC3B Polyclonal Antibody, Cy7 Conjugated (bs-2912R-Cy7) react with?
Tested reactivity: Mouse, Rat, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken, Zebrafish.
How should bs-2912R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LC3B Polyclonal Antibody, Cy7 Conjugated (bs-2912R-Cy7)?
KLH conjugated synthetic peptide derived from human MAP1A/MAP1B LC3 B Immunogen range: 21-121/121.
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-2912R-Cy7)?
Cy7 on bs-2912R-Cy7: excitation / emission 743nm/767nm.