EIF5 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2913r-pe |
| Product Name | EIF5 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EIF5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1983 |
| Synonyms | E 5; E 5A; Eukaryotic initiation factor 5; Eukaryotic translation initiation factor 5; 5_HUMAN. |
| Background | EIF5, Eukaryotic initiation factor 5, catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is EIF5 Polyclonal Antibody, PE Conjugated (bs-2913R-PE) validated for, and at what dilution?
bs-2913R-PE is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EIF5 Polyclonal Antibody, PE Conjugated (bs-2913R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-2913R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EIF5 Polyclonal Antibody, PE Conjugated (bs-2913R-PE)?
KLH conjugated synthetic peptide derived from human EIF5
What are the excitation and emission wavelengths of the PE conjugate (bs-2913R-PE)?
PE on bs-2913R-PE: excitation / emission 496,564nm/578nm.