SPINK1 Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-2917R |
| Product Name | SPINK1 Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This protein is known to be similar in amino acid sequence to SPINK3 and thus may cross-react with this protein. |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat SPINK1 |
| Immunogen Range | 31-79/79 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 24833 |
| Swiss Prot | P09655 |
| Subcellular location | Secreted |
| Synonyms | Tilp; Psti-1; Spink1; Serine protease inhibitor Kazal-type 1; Cholecystokinin-releasing peptide; Monitor peptide; Pancreatic secretory trypsin inhibitor; PSTI-I |
| Background | Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:3202973, PubMed:3597401). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity). In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production (By similarity). |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SPINK1 Polyclonal Antibody (bs-2917R) validated for, and at what dilution?
bs-2917R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-2917R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SPINK1 Polyclonal Antibody (bs-2917R)?
KLH conjugated synthetic peptide derived from rat SPINK1 Immunogen range: 31-79/79.