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SPINK1 Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Rat
Overview
Catalog # bs-2917R-APC-Cy7
Product Name SPINK1 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This protein is known to be similar in amino acid sequence to SPINK3 and thus may cross-react with this protein.
Predicted Reactivity Rat
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat SPINK1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 24833
Swiss Prot P09655
Subcellular location Secreted
Synonyms Tilp; Psti-1; Spink1; Serine protease inhibitor Kazal-type 1; Cholecystokinin-releasing peptide; Monitor peptide; Pancreatic secretory trypsin inhibitor; PSTI-I
Background Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:3202973, PubMed:3597401). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity). In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production (By similarity).
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SPINK1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-2917R-APC-Cy7) validated for, and at what dilution?

bs-2917R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-2917R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPINK1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-2917R-APC-Cy7)?

KLH conjugated synthetic peptide derived from rat SPINK1

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-2917R-APC-Cy7)?

APC-Cy7 on bs-2917R-APC-Cy7: excitation / emission 650nm/780nm.