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Collagen XI alpha 2 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-2924R-PerCP
Product Name Collagen XI alpha 2 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Pig, Horse, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human COL11A2 COLFI domain.
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1302
Swiss Prot P13942
Subcellular location Secreted, Extracellular matrix
Synonyms HKE5; PARP; STL3; FBCG2; DFNA13; DFNB53; Collagen alpha-2(XI) chain; COL11A2
Background May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Collagen XI alpha 2 Polyclonal Antibody, PerCP Conjugated (bs-2924R-PerCP) validated for, and at what dilution?

bs-2924R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Collagen XI alpha 2 Polyclonal Antibody, PerCP Conjugated (bs-2924R-PerCP) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Pig, Horse, Rabbit.

How should bs-2924R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Collagen XI alpha 2 Polyclonal Antibody, PerCP Conjugated (bs-2924R-PerCP)?

KLH conjugated synthetic peptide derived from human COL11A2 COLFI domain.

What are the excitation and emission wavelengths of the PerCP conjugate (bs-2924R-PerCP)?

PerCP on bs-2924R-PerCP: excitation / emission 482nm/677nm.