Clathrin heavy chain Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2932R-Cy3 |
| Product Name | Clathrin heavy chain Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Clathrin heavy chain. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1213, 8218 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CHC17; Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain; Clathrin heavy chain like 1; Clathrin heavy chain on chromosome 17; Clathrin heavy polypeptide; CLH 17; CLH 22; CLH-17; CLH1_HUMAN; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034. |
| Background | Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Clathrin heavy chain Polyclonal Antibody, Cy3 Conjugated (bs-2932R-Cy3) validated for, and at what dilution?
bs-2932R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Clathrin heavy chain Polyclonal Antibody, Cy3 Conjugated (bs-2932R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Rabbit.
How should bs-2932R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Clathrin heavy chain Polyclonal Antibody, Cy3 Conjugated (bs-2932R-Cy3)?
KLH conjugated synthetic peptide derived from human Clathrin heavy chain.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2932R-Cy3)?
Cy3 on bs-2932R-Cy3: excitation / emission 512,550nm/570,615nm.