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Clathrin heavy chain Polyclonal Antibody, FITC Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-2932R-FITC
Product Name Clathrin heavy chain Polyclonal Antibody, FITC Conjugated
Applications
WB
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Pig, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Clathrin heavy chain.
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1213, 8218
Subcellular location Cytoplasm, Cell membrane
Synonyms CHC17; Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain; Clathrin heavy chain like 1; Clathrin heavy chain on chromosome 17; Clathrin heavy polypeptide; CLH 17; CLH 22; CLH-17; CLH1_HUMAN; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034.
Background Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Clathrin heavy chain Polyclonal Antibody, FITC Conjugated (bs-2932R-FITC) validated for, and at what dilution?

bs-2932R-FITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Clathrin heavy chain Polyclonal Antibody, FITC Conjugated (bs-2932R-FITC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Rabbit.

How should bs-2932R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Clathrin heavy chain Polyclonal Antibody, FITC Conjugated (bs-2932R-FITC)?

KLH conjugated synthetic peptide derived from human Clathrin heavy chain.

What are the excitation and emission wavelengths of the FITC conjugate (bs-2932R-FITC)?

FITC on bs-2932R-FITC: excitation / emission 494nm/518nm.