RAB14 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-2933R-BF750 |
| Product Name | RAB14 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAB-14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51552 |
| Swiss Prot | P61106 |
| Subcellular location | Cytoplasm |
| Synonyms | FBP; RAB-14; Ras-related protein Rab-14; RAB14 |
| Background | Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes (By similarity). Regulates, together with its guanine nucleotide exchange factor DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RAB14 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-2933R-BF750) validated for, and at what dilution?
bs-2933R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-2933R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAB14 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-2933R-BF750)?
KLH conjugated synthetic peptide derived from human RAB-14
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-2933R-BF750)?
AbBy Fluor-750 on bs-2933R-BF750: excitation / emission 749nm/775nm.