Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

RASSF2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse brain lysates Lane 2: mouse kidney lysates probed with Anti RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 36kD. Observed band size: 36kD.
Lane 1: mouse brain lysates Lane 2: mouse kidney lysates probed with Anti RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 36kD. Observed band size: 36kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Pig
  • Horse
Overview
Catalog # bs-2939R
Product Name RASSF2 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This protein may cross-react with RASSF6 in mouse, dog, pig and rat in the samples in which they are both found, which are placenta, lung, and brain.
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Pig, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RASSF2
Immunogen Range 201-300/326
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 9770
Swiss Prot P50749
Subcellular location Nucleus
Synonyms CENP-34; RASFADIN; Ras association domain-containing protein 2; RASSF2; KIAA0168
Background Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is RASSF2 Polyclonal Antibody (bs-2939R) validated for, and at what dilution?

bs-2939R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RASSF2 Polyclonal Antibody (bs-2939R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Pig, Horse.

How should bs-2939R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RASSF2 Polyclonal Antibody (bs-2939R)?

KLH conjugated synthetic peptide derived from human RASSF2 Immunogen range: 201-300/326.

Search our catalog of hundreds of thousands of products