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RASSF2 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Pig
  • Horse
Overview
Catalog # bs-2939R-BF350
Product Name RASSF2 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This protein may cross-react with RASSF6 in mouse, dog, pig and rat in the samples in which they are both found, which are placenta, lung, and brain.
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Pig, Horse
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RASSF2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9770
Swiss Prot P50749
Subcellular location Nucleus
Synonyms CENP-34; RASFADIN; Ras association domain-containing protein 2; RASSF2; KIAA0168
Background Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is RASSF2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-2939R-BF350) validated for, and at what dilution?

bs-2939R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RASSF2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-2939R-BF350) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Pig, Horse.

How should bs-2939R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RASSF2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-2939R-BF350)?

KLH conjugated synthetic peptide derived from human RASSF2

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-2939R-BF350)?

AbBy Fluor-350 on bs-2939R-BF350: excitation / emission 346nm/442nm.