SEMA6D Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2943R-RBITC |
| Product Name | SEMA6D Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SEMA6D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80031 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | SEM6D_HUMAN; KIAA1479; Q8NFY4; Sema domain, transmembrane domain TM, and cytoplasmic domain, semaphorin 6D; Semaphorin 6D. |
| Background | Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq, Nov 2010] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SEMA6D Polyclonal Antibody, RBITC Conjugated (bs-2943R-RBITC) validated for, and at what dilution?
bs-2943R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SEMA6D Polyclonal Antibody, RBITC Conjugated (bs-2943R-RBITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse.
How should bs-2943R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SEMA6D Polyclonal Antibody, RBITC Conjugated (bs-2943R-RBITC)?
KLH conjugated synthetic peptide derived from human SEMA6D
What are the excitation and emission wavelengths of the RBITC conjugate (bs-2943R-RBITC)?
RBITC on bs-2943R-RBITC: excitation / emission 540nm/625nm.