Syncytin 1 Polyclonal Antibody, PE-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2962R-PE-Cy7 |
| Product Name | Syncytin 1 Polyclonal Antibody, PE-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Syncytin 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 30816 |
| Swiss Prot | Q9UQF0 |
| Subcellular location | Cell membrane, Extracellular matrix |
| Synonyms | ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENV; Syncytin-1; Endogenous retrovirus group W member 1; Env-W; Envelope polyprotein gPr73; Enverin; HERV-7q Envelope protein; HERV-W envelope protein; HERV-W_7q21.2 provirus ancestral Env polyprotein; Syncytin; ERVW-1 |
| Background | This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. May induce fusion through binding of SLC1A4 and SLC1A5 (PubMed:10708449, PubMed:12050356, PubMed:23492904). Endogenous envelope proteins may have kept, lost or modified their original function during evolution. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein (SU) mediates receptor recognition, while the transmembrane protein (TM) acts as a class I viral fusion protein. The protein may have at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Syncytin 1 Polyclonal Antibody, PE-Cy7 Conjugated (bs-2962R-PE-Cy7) validated for, and at what dilution?
bs-2962R-PE-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Syncytin 1 Polyclonal Antibody, PE-Cy7 Conjugated (bs-2962R-PE-Cy7) react with?
Tested reactivity: Human, Mouse.
How should bs-2962R-PE-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Syncytin 1 Polyclonal Antibody, PE-Cy7 Conjugated (bs-2962R-PE-Cy7)?
KLH conjugated synthetic peptide derived from human Syncytin 1
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-2962R-PE-Cy7)?
PE-Cy7 on bs-2962R-PE-Cy7: excitation / emission 488nm/780nm.