SMAD1 + SMAD5 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2973R-RBITC-100uL |
| Product Name | SMAD1 + SMAD5 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Specificity | The sequence used to produce this antibody is conserved in Smad 1, 2, 3 and 9 and expected to cross react due to sequence homology. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SMAD5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4090 |
| Swiss Prot | Q99717 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DWFC; JV5-1; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; SMAD family member 5; SMAD 5; Smad5; hSmad5 |
| Background | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | ICC1:100 |
Frequently asked questions
Which applications is SMAD1 + SMAD5 Polyclonal Antibody, RBITC Conjugated (bs-2973R-RBITC) validated for, and at what dilution?
bs-2973R-RBITC is validated for WB (1:500-2000); IF(ICC) (1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMAD1 + SMAD5 Polyclonal Antibody, RBITC Conjugated (bs-2973R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-2973R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMAD1 + SMAD5 Polyclonal Antibody, RBITC Conjugated (bs-2973R-RBITC)?
KLH conjugated synthetic peptide derived from human SMAD5
What are the excitation and emission wavelengths of the RBITC conjugate (bs-2973R-RBITC)?
RBITC on bs-2973R-RBITC: excitation / emission 540nm/625nm.