SMAD1 + SMAD5 Polyclonal Antibody

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with SMAD1 + SMAD5 Polyclonal Antibody, Unconjugated (bs-2973R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with SMAD1 + SMAD5 Polyclonal Antibody, Unconjugated (bs-2973R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-2973R
Product Name	SMAD1 + SMAD5 Polyclonal Antibody
Applications	WB IHC-P IHC-F IF ICC/IF
Specificity	The sequence used to produce this antibody is conserved in Smad 1, 2, 3 and 9 and expected to cross react due to sequence homology.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SMAD5
Immunogen Range	360-410/465
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4090
Swiss Prot	Q99717
Subcellular location	Cytoplasm, Nucleus
Synonyms	DWFC; JV5-1; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; SMAD family member 5; SMAD 5; Smad5; hSmad5
Background	Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Application Dilution
WB	1:500-2000
IHC-P	1:100-500
IHC-F	IFICC1:100
IF
ICC/IF