SMAD1 + SMAD5 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-2973R |
| Product Name | SMAD1 + SMAD5 Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
ICC/IF
|
| Specificity | The sequence used to produce this antibody is conserved in Smad 1, 2, 3 and 9 and expected to cross react due to sequence homology. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SMAD5 |
| Immunogen Range | 360-410/465 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 4090 |
| Swiss Prot | Q99717 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DWFC; JV5-1; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; SMAD family member 5; SMAD 5; Smad5; hSmad5 |
| Background | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | 1:100-500 |
| IHC-F | IFICC1:100 |
| IF | |
| ICC/IF | |
Frequently asked questions
Which applications is SMAD1 + SMAD5 Polyclonal Antibody (bs-2973R) validated for, and at what dilution?
bs-2973R is validated for WB (1:500-2000); IHC-P (1:100-500); IF(ICC) (1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMAD1 + SMAD5 Polyclonal Antibody (bs-2973R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-2973R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMAD1 + SMAD5 Polyclonal Antibody (bs-2973R)?
KLH conjugated synthetic peptide derived from human SMAD5 Immunogen range: 360-410/465.