Help

Factor VIII Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-2974R-APC-Cy7
Product Name Factor VIII Polyclonal Antibody, APC-Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Rabbit
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Factor VIII
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2157
Swiss Prot P00451
Subcellular location Secreted, Extracellular matrix
Synonyms AHF; F8B; F8C; HEMA; FVIII; DXS1253E; Coagulation factor VIII; Antihemophilic factor; Procoagulant component; F8
Background Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Factor VIII Polyclonal Antibody, APC-Cy7 Conjugated (bs-2974R-APC-Cy7) validated for, and at what dilution?

bs-2974R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Factor VIII Polyclonal Antibody, APC-Cy7 Conjugated (bs-2974R-APC-Cy7) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Rabbit.

How should bs-2974R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Factor VIII Polyclonal Antibody, APC-Cy7 Conjugated (bs-2974R-APC-Cy7)?

KLH conjugated synthetic peptide derived from human Factor VIII

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-2974R-APC-Cy7)?

APC-Cy7 on bs-2974R-APC-Cy7: excitation / emission 650nm/780nm.