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Noggin Polyclonal Antibody, PE-Cy7 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-2975R-PE-Cy7
Product Name Noggin Polyclonal Antibody, PE-Cy7 Conjugated
Applications
WB FCM
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation PE-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Noggin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9241
Swiss Prot Q13253
Subcellular location Secreted
Synonyms NOG; NOGG_HUMAN; Noggin; SYM 1; SYM1; Symphalangism 1 proximal; Synostoses multiple syndrome 1; SYNS 1; SYNS1.
Background Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 (PubMed:21976273).
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is Noggin Polyclonal Antibody, PE-Cy7 Conjugated (bs-2975R-PE-Cy7) validated for, and at what dilution?

bs-2975R-PE-Cy7 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Noggin Polyclonal Antibody, PE-Cy7 Conjugated (bs-2975R-PE-Cy7) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-2975R-PE-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Noggin Polyclonal Antibody, PE-Cy7 Conjugated (bs-2975R-PE-Cy7)?

KLH conjugated synthetic peptide derived from human Noggin

What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-2975R-PE-Cy7)?

PE-Cy7 on bs-2975R-PE-Cy7: excitation / emission 488nm/780nm.