GGT1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2976R-RBITC-100uL |
| Product Name | GGT1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GGT1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2678 |
| Swiss Prot | P19440 |
| Subcellular location | Cell membrane |
| Synonyms | GGT; GTG; CD224; GGT 1; D22S672; D22S732; Gamma-glutamyltranspeptidase 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1; Leukotriene-C4 hydrolase; GGT1 |
| Background | Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide, cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Isoform 3 seems to be inactive. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is GGT1 Polyclonal Antibody, RBITC Conjugated (bs-2976R-RBITC) validated for, and at what dilution?
bs-2976R-RBITC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GGT1 Polyclonal Antibody, RBITC Conjugated (bs-2976R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-2976R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GGT1 Polyclonal Antibody, RBITC Conjugated (bs-2976R-RBITC)?
KLH conjugated synthetic peptide derived from human GGT1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-2976R-RBITC)?
RBITC on bs-2976R-RBITC: excitation / emission 540nm/625nm.