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ADM2 Polyclonal Antibody

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat small intestine labeled with Anti-ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat small intestine labeled with Anti-ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADM2 Polyclonal Antibody, Unconjugated (bs-2985R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Colon Lysates; Lane 2: Human U-2 OS cell Lysates. Probed with ADM2 polyclonal Antibody, unconjugated (bs-2985R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Colon Lysates; Lane 2: Human U-2 OS cell Lysates. Probed with ADM2 polyclonal Antibody, unconjugated (bs-2985R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-2985R
Product Name ADM2 Polyclonal Antibody
Applications
ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Pig, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ADM2/ Intermedin
Immunogen Range 75-148/148
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 79924
Swiss Prot Q7Z4H4
Subcellular location Secreted
Synonyms AM2; dJ579N16.4; ADM2; Intermedin
Background IMDL and IMDS may play a role as physiological regulators of gastrointestinal, cardiovascular bioactivities mediated by the CALCRL/RAMPs receptor complexes. Activates the cAMP-dependent pathway.
Application Dilution
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ADM2 Polyclonal Antibody (bs-2985R) validated for, and at what dilution?

bs-2985R is validated for ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADM2 Polyclonal Antibody (bs-2985R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse, Rabbit.

How should bs-2985R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADM2 Polyclonal Antibody (bs-2985R)?

KLH conjugated synthetic peptide derived from human ADM2/ Intermedin Immunogen range: 75-148/148.

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