Mitofusin 2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2988r-cy55-100uL |
| Product Name | Mitofusin 2 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Mitofusin 2 |
| Immunogen Range | 1-120/757 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9927 |
| Swiss Prot | O95140 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CMT2A; CMT2A2; CMT2A2A; CMT2A2B; CPRP1; HMSN6A; HSG; MARF; MSL; D630023P19Rik; Fzo; MFN2_HUMAN; MFN2; Transmembrane GTPase MFN2; 3.6.5.-; KIAA0214; MFN2_MOUSE; Hypertension-related protein 1; Mitochondrial assembly regulatory factor (HSG protein); MFN2_RAT; Mitochondrial transmembrane GTPase FZO1A (Protein HSG); Fzo1a; mitofusin 2 |
| Background | Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is Mitofusin 2 Polyclonal Antibody, Cy5.5 Conjugated (BS-2988R-CY5-5) validated for, and at what dilution?
BS-2988R-CY5-5 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mitofusin 2 Polyclonal Antibody, Cy5.5 Conjugated (BS-2988R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken.
How should BS-2988R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Mitofusin 2 Polyclonal Antibody, Cy5.5 Conjugated (BS-2988R-CY5-5)?
KLH conjugated synthetic peptide derived from human Mitofusin 2 Immunogen range: 1-120/757.
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-2988R-CY5-5)?
Cy5.5 on BS-2988R-CY5-5: excitation / emission 675nm/694nm.