MLK3 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2992R-BF350 |
| Product Name | MLK3 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Due to the similarity of this protein to MLK1 2 and 4, there is a chance that this antibody will cross-react based on homology. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MLK3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4296 |
| Swiss Prot | Q16584 |
| Subcellular location | Cytoplasm |
| Synonyms | MLK3; PTK1; SPRK; MLK-3; MEKK11; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase; MAP3K11 |
| Background | Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MLK3 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-2992R-BF350) validated for, and at what dilution?
bs-2992R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MLK3 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-2992R-BF350) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Horse.
How should bs-2992R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MLK3 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-2992R-BF350)?
KLH conjugated synthetic peptide derived from human MLK3
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-2992R-BF350)?
AbBy Fluor-350 on bs-2992R-BF350: excitation / emission 346nm/442nm.