JNK3/MAPK10 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2997R-BF488 |
| Product Name | JNK3/MAPK10 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human JNK3/MAPK10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5602 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | cJun N terminal kinase 3; MAP kinase p49 3F12; Stress activated protein kinase JNK3; c Jun kinase 3; JNK3; JNK3A; MAP kinase; mitogen activated protein kinase 10; p493F12; p54bSAPK; PRKM10; protein kinase mitogen activated 10 ; stress activated protein kinase beta. |
| Background | MAPK10 (JNK3) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuron-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways of neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 (CDK5) can phosphorylate and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is JNK3/MAPK10 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-2997R-BF488) validated for, and at what dilution?
bs-2997R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does JNK3/MAPK10 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-2997R-BF488) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Chicken, Rabbit.
How should bs-2997R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise JNK3/MAPK10 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-2997R-BF488)?
KLH conjugated synthetic peptide derived from human JNK3/MAPK10
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-2997R-BF488)?
AbBy Fluor-488 on bs-2997R-BF488: excitation / emission 499nm/519nm.