phospho-14-3-3 protein zeta (Ser58) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3000R-BF750 |
| Product Name | phospho-14-3-3 protein zeta (Ser58) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
FCM
|
| Specificity | This antibody will react with all 14-3-3 proteins containing the conserved motif RS(S-p)WR. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human 14-3-3 protein zeta/delta around the phosphorylation site of Ser58 [RS(p-S)WR] |
| Modification Site | Ser58 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7534, 7529, 10971 |
| Subcellular location | Cytoplasm |
| Synonyms | YWHAEphospho S58; YWHAZphospho S58; 14 3 3; 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; 14-3-3 protein beta/alpha; KCIP 1; Protein 1054; Protein kinase C inhibitor protein 1; YWHAB; YWHAZ; GW128; HS1; KCIP-1; YWHAE; YWHAA; 1433Z_HUMAN; Full=14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1. |
| Background | Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is phospho-14-3-3 protein zeta (Ser58) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3000R-BF750) validated for, and at what dilution?
bs-3000R-BF750 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-14-3-3 protein zeta (Ser58) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3000R-BF750) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Horse, Chicken, Rabbit.
How should bs-3000R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-14-3-3 protein zeta (Ser58) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3000R-BF750)?
KLH conjugated synthetic phosphopeptide derived from human 14-3-3 protein zeta/delta around the phosphorylation site of Ser58 [RS(p-S)WR]
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-3000R-BF750)?
AbBy Fluor-750 on bs-3000R-BF750: excitation / emission 749nm/775nm.