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phospho-ADRB2 (Ser346) Polyclonal Antibody, Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Sheep
  • Horse
Overview
Catalog # bs-3002R-Cy7
Product Name phospho-ADRB2 (Ser346) Polyclonal Antibody, Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Sheep, Horse
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human ADRB2 around the phosphorylation site of Ser346
Modification Site Ser346
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 154
Swiss Prot P07550
Subcellular location Cytoplasm, Cell membrane
Synonyms BAR; B2AR; ADRBR; ADRB2R; BETA2AR; Beta-2 adrenergic receptor; Beta-2 adrenoreceptor; Beta-2 adrenoceptor; ADRB2
Background Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is phospho-ADRB2 (Ser346) Polyclonal Antibody, Cy7 Conjugated (bs-3002R-Cy7) validated for, and at what dilution?

bs-3002R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-ADRB2 (Ser346) Polyclonal Antibody, Cy7 Conjugated (bs-3002R-Cy7) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Sheep, Horse.

How should bs-3002R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-ADRB2 (Ser346) Polyclonal Antibody, Cy7 Conjugated (bs-3002R-Cy7)?

KLH conjugated synthetic phosphopeptide derived from human ADRB2 around the phosphorylation site of Ser346

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-3002R-Cy7)?

Cy7 on bs-3002R-Cy7: excitation / emission 743nm/767nm.