phospho-AR/Androgen receptor (Ser597) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3004R-BF750-100uL |
| Product Name | phospho-AR/Androgen receptor (Ser597) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat, Bovine |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser597 [CA(p-S)RN] |
| Modification Site | Ser597 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 367 |
| Synonyms | Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; AR; DHTR; NR3C4 |
| Background | Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is phospho-AR/Androgen receptor (Ser597) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3004R-BF750) validated for, and at what dilution?
bs-3004R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-AR/Androgen receptor (Ser597) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3004R-BF750) react with?
Tested reactivity: Human, Mouse, Rat, Bovine.
How should bs-3004R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-AR/Androgen receptor (Ser597) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3004R-BF750)?
KLH conjugated synthetic phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser597 [CA(p-S)RN]
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-3004R-BF750)?
AbBy Fluor-750 on bs-3004R-BF750: excitation / emission 749nm/775nm.