phospho-Bax (Ser184) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3010R-100uL |
| Product Name | phospho-Bax (Ser184) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Bax around the phosphorylation site of Ser184 |
| Modification Site | Ser184 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 581 |
| Swiss Prot | Q07812 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX |
| Background | Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | FCM1:20-100 |
| IHC-F | IHC-P1:200-400 |
| IF | |
| Flow-Cyt | |
Frequently asked questions
Which applications is phospho-Bax (Ser184) Polyclonal Antibody (bs-3010R) validated for, and at what dilution?
bs-3010R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Bax (Ser184) Polyclonal Antibody (bs-3010R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig.
How should bs-3010R be stored?
Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Bax (Ser184) Polyclonal Antibody (bs-3010R)?
KLH conjugated synthetic phosphopeptide derived from human Bax around the phosphorylation site of Ser184