Bax (Ser184) Polyclonal Antibody

$Lane 1:mouse brain lysates probed with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37\u02daC.$

Lane 1:mouse brain lysates probed with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37\u02daC.

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 followed by Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei\\n$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 followed by Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei\\n

$Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
FCM

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig

Overview
Catalog #	bs-3010R
Product Name	Bax (Ser184) Polyclonal Antibody
Applications	WB, ELISA, FCM
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Bax around the phosphorylation site of Ser184
Modification Site	Ser184
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition	Store at -20°C for 12 months..
Target
Gene ID	581
Swiss Prot	Q07812
Subcellular location	Cytoplasm, Cell membrane
Synonyms	BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX
Background	Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Application Dilution
WB	1:300-1000
ELISA	1:500-1000
FCM	1:20-100

Applications

Reactivity

Predicted Reactivity

Search our catalog of hundreds of thousands of products