Bax (Ser184) Polyclonal Antibody

$Lane 1:mouse brain lysates probed with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37\u02daC.$

Lane 1:mouse brain lysates probed with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37\u02daC.

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 followed by Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei\\n$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 followed by Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei\\n

$Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-3010R
Product Name	Bax (Ser184) Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Bax around the phosphorylation site of Ser184
Immunogen Range	179-192/192
Modification Site	Ser184
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID	581
Swiss Prot	Q07812
Subcellular location	Cytoplasm, Cell membrane
Synonyms	BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX
Background	Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Application Dilution
WB	1:300-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

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