phospho-Bax (Ser184) Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3010R-PE-Cy5-100uL |
| Product Name | phospho-Bax (Ser184) Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Bax around the phosphorylation site of Ser184 |
| Modification Site | Ser184 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 581 |
| Swiss Prot | Q07812 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX |
| Background | Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-Bax (Ser184) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3010R-PE-Cy5) validated for, and at what dilution?
bs-3010R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Bax (Ser184) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3010R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig.
How should bs-3010R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Bax (Ser184) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3010R-PE-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human Bax around the phosphorylation site of Ser184
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3010R-PE-Cy5)?
PE-Cy5 on bs-3010R-PE-Cy5: excitation / emission 488nm/670nm.