phospho-BRAF (Ser365) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3013R-Cy5 |
| Product Name | phospho-BRAF (Ser365) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phophorylation site is homologous to that of Ser348 in Mouse and Ser347 in Rat. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human B Raf around the phosphorylation site of Ser365 |
| Modification Site | Ser365 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 673 |
| Swiss Prot | P15056 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | NS7; BRAF1; RAFB1; B-RAF1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF |
| Background | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-BRAF (Ser365) Polyclonal Antibody, Cy5 Conjugated (bs-3013R-Cy5) validated for, and at what dilution?
bs-3013R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-BRAF (Ser365) Polyclonal Antibody, Cy5 Conjugated (bs-3013R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-3013R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-BRAF (Ser365) Polyclonal Antibody, Cy5 Conjugated (bs-3013R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human B Raf around the phosphorylation site of Ser365
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3013R-Cy5)?
Cy5 on bs-3013R-Cy5: excitation / emission 625,650nm/670nm.