phospho-eIF4EBP1 (Thr37 + Thr46) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3019R-Biotin |
| Product Name | phospho-eIF4EBP1 (Thr37 + Thr46) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, Goat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human 4EBP1 around the phosphorylation site of Thr37/46 |
| Modification Site | Thr37 + Thr46 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 1978 |
| Swiss Prot | Q13541 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | BP-1; 4EBP1; 4E-BP1; PHAS-I; Eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; EIF4EBP1 |
| Background | Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-eIF4EBP1 (Thr37 + Thr46) Polyclonal Antibody, Biotin Conjugated (bs-3019R-Biotin) validated for, and at what dilution?
bs-3019R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-eIF4EBP1 (Thr37 + Thr46) Polyclonal Antibody, Biotin Conjugated (bs-3019R-Biotin) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, Goat.
How should bs-3019R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-eIF4EBP1 (Thr37 + Thr46) Polyclonal Antibody, Biotin Conjugated (bs-3019R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human 4EBP1 around the phosphorylation site of Thr37/46