phospho-p53BP1 (Ser25 + Ser29) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3020R |
| Product Name | phospho-p53BP1 (Ser25 + Ser29) Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylated site is the same in Mouse and homologous to p53BP1 Ser30 + Ser34 in Rat. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human p53BP1 around the phosphorylation site of Ser25/29 |
| Modification Site | Ser25 + Ser29 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7158 |
| Swiss Prot | Q12888 |
| Subcellular location | Nucleus |
| Synonyms | p202; 53BP1; Tumor suppressor p53-binding protein 1; p53-binding protein 1; p53BP1; TP53BP1 |
| Background | Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-p53BP1 (Ser25 + Ser29) Polyclonal Antibody (bs-3020R) validated for, and at what dilution?
bs-3020R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-p53BP1 (Ser25 + Ser29) Polyclonal Antibody (bs-3020R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Rabbit.
How should bs-3020R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-p53BP1 (Ser25 + Ser29) Polyclonal Antibody (bs-3020R)?
KLH conjugated synthetic phosphopeptide derived from human p53BP1 around the phosphorylation site of Ser25/29