Phospho-ATP1A1 (Tyr10) Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3034R-Cy5-100uL |
| Product Name | Phospho-ATP1A1 (Tyr10) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Na,K-ATPase alpha-1 around the phosphorylation site of tyrosine 10 |
| Modification Site | Tyr10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 24211 |
| Swiss Prot | P06685 |
| Subcellular location | Cell membrane |
| Synonyms | Nkaa1b; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1; Atp1a1 |
| Background | This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-ATP1A1 (Tyr10) Polyclonal Antibody, Cy5 Conjugated (bs-3034R-Cy5) validated for, and at what dilution?
bs-3034R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3034R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-ATP1A1 (Tyr10) Polyclonal Antibody, Cy5 Conjugated (bs-3034R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human Na,K-ATPase alpha-1 around the phosphorylation site of tyrosine 10
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3034R-Cy5)?
Cy5 on bs-3034R-Cy5: excitation / emission 625,650nm/670nm.