Phospho-CD19 (Tyr531) Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3065R-PE-100uL |
| Product Name | Phospho-CD19 (Tyr531) Polyclonal Antibody, PE Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CD19 around the phosphorylation site of Tyr531 [DS(p-Y)EN] |
| Modification Site | Tyr531 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 930 |
| Swiss Prot | P15391 |
| Subcellular location | Cell membrane |
| Synonyms | B4; CVID3; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19 |
| Background | Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is Phospho-CD19 (Tyr531) Polyclonal Antibody, PE Conjugated (bs-3065R-PE) validated for, and at what dilution?
bs-3065R-PE is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CD19 (Tyr531) Polyclonal Antibody, PE Conjugated (bs-3065R-PE) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-3065R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CD19 (Tyr531) Polyclonal Antibody, PE Conjugated (bs-3065R-PE)?
KLH conjugated synthetic phosphopeptide derived from human CD19 around the phosphorylation site of Tyr531 [DS(p-Y)EN]
What are the excitation and emission wavelengths of the PE conjugate (bs-3065R-PE)?
PE on bs-3065R-PE: excitation / emission 496,564nm/578nm.