Phospho-CENPA (Ser7) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3066R-Cy3-100uL |
| Product Name | Phospho-CENPA (Ser7) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CENP-A around the phosphorylation site of Ser7 [RR(p-S)RK] |
| Modification Site | Ser7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1058 |
| Subcellular location | Nucleus |
| Synonyms | CENP A; CENP-A; Centromere autoantigen A; Centromere protein A 17kDa; centromere protein A; centromeric protein A; Histone H3 like centromeric protein A. |
| Background | Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-CENPA (Ser7) Polyclonal Antibody, Cy3 Conjugated (bs-3066R-Cy3) validated for, and at what dilution?
bs-3066R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CENPA (Ser7) Polyclonal Antibody, Cy3 Conjugated (bs-3066R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit.
How should bs-3066R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CENPA (Ser7) Polyclonal Antibody, Cy3 Conjugated (bs-3066R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human CENP-A around the phosphorylation site of Ser7 [RR(p-S)RK]
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3066R-Cy3)?
Cy3 on bs-3066R-Cy3: excitation / emission 512,550nm/570,615nm.