Phospho-Bcr (Tyr177) Polyclonal Antibody, APC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3067R-APC-100uL |
| Product Name | Phospho-Bcr (Tyr177) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Pig, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Bcr around the phosphorylation site of Tyr177 [PF(p-Y)VN] |
| Modification Site | Tyr177 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 613 |
| Subcellular location | Cell membrane |
| Synonyms | Bcr phospho Y177; BcrTyr177; ALL; BCR 1; BCR/ABL FUSION GENE INCLUDED; BCR/FGFR1 FUSION GENE INCLUDED; BCR/PDGFRA FUSION GENE INCLUDED; BCR1; breakpoint cluster region; CML; PHL; BCR_HUMAN. |
| Background | The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Bcr (Tyr177) Polyclonal Antibody, APC Conjugated (bs-3067R-APC) validated for, and at what dilution?
bs-3067R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3067R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Bcr (Tyr177) Polyclonal Antibody, APC Conjugated (bs-3067R-APC)?
KLH conjugated synthetic phosphopeptide derived from human Bcr around the phosphorylation site of Tyr177 [PF(p-Y)VN]
What are the excitation and emission wavelengths of the APC conjugate (bs-3067R-APC)?
APC on bs-3067R-APC: excitation / emission 650nm/660nm.